Midbody Isolation (Skop, 2004)
| skopmidbodyisolation7-2008.pdf |
C. elegans Blastomere Culture Media (Shelton's Media)(from Skop, 2000)
| ahna_embryonic_growth_medium.docx |
| sheltons_growth_media_2.docx |
Antibody Staining for Embryos (Skop, 1998+)
Note: You use RT (room temp) Methanol so that the embryos don't shrink. Cold MeOH causes the embryos to shrink considerably.
| ahnas_antibody_staining_updated.doc |
| skopactin_tubulin_staining.docx |
| phalloidin_staining.doc |
Antibody staining for membrane proteins(Gonczy, 1999)
| gonczyantibodystainingprotocol1999.doc |
Skop Lab Bombardment Protocol (Nakayama, Shivas, et al, 2009)
| bombardment.pdf |